E08L0268 Canine Lipopolysaccharides/lipooligosaccharide ELISA kit
The Canine Lipopolysaccharides/lipooligosaccharide ELISA kit can be used to identify samples from the Canine species. Lipopolysaccharides/lipooligosaccharide can also be called Lipoglycans, Lipooligosaccharide, Lipo-Oligosaccharide, Endotoxin, LPS/LOS.
Product Information | |
Cat. No. | E08L0268 |
Product Name | Canine Lipopolysaccharides/lipooligosaccharide ELISA kit |
Species | Canine |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LPS/LOS ELISA kit uses an anti-LPS/LOS antibody and an LPS/LOS-HRP conjugate in a competitive enzyme immunoassay method. LPS/LOS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LPS/LOS antibody binding site between LPS/LOS from samples and LPS/LOS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LPS/LOS. Since the number of sites is limited, as more sites are occupied by LPS/LOS from the sample, fewer sites are left to bind LPS/LOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LPS/LOS concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LPS/LOS and analogues was observed. | |
E08L0268 has been referenced in the below publications：
Toll-like receptor mediated activation is possibly involved in immunoregulating properties of cow’s milk hydrolysates.
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